
A PCR detection system with 96-well block and 2-color detection plus FRET. Includes CFX Duet base unit and cables.
Overview
The CFX Duet Real-Time PCR System makes the power and consistency of Bio-Rad qPCR available to almost any lab. The CFX Duet System is the fresh, modern system for small and more economical labs with its improved thermal performance and proprietary, accurate optical shuttle system. The new compact, cost-effective system will enable you to make discoveries in the convenience of your own laboratory without the need for sharing with your neighbors or other departments.
Features and Benefits
Quick system set up — easy installation and factory-calibrated optics
Our best thermal uniformity and accuracy to date
Uniform optical system free of regular calibration requirements
Audit and e-signature compatibility with CFX Maestro Security Edition Software
Eliminates the need for a reference/normalizing dye, the CFX Duet System can perform duplex assays including genotyping
Specifications
| Thermal Cycler | |
| Maximum ramp rate, °C/sec | 5 |
| Average ramp rate, °C/sec | 3.3 |
| Heating and cooling method | Peltier |
| Lid, °C | 30–110 |
| Temperature | |
| Accuracy, °C | ±0.2 of programmed target at 90°C |
| Uniformity, °C | ±0.3 well-to-well after 10 sec of arrival at target |
| Gradient | |
| Operational range, °C | 30–100 |
| Programmable span, °C | 1–24 |
| Optical Detection | |
| Excitation | 3 filtered LEDs |
| Detection | 3 filtered photodiodes |
| Range of excitation/emission wavelengths, nm | 450–580 |
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA |
| Dynamic range | 10 orders of magnitude |
| Scan Time | |
| All channels, sec | 12 |
| FAM/SYBR® Green only, sec | 3 |
| Software | |
| Operating systems | Windows 10 and up |
| Multiplex analysis | Up to 2 targets per well |
| System | |
| Sample capacity, wells | 96 |
| Sample size, µl | 1–50 (10–50 recommended) |
| Communication interface | USB 2.0 |
| Electrical approvals | IEC, CE |
| Dimensions (W x D x H), cm/in | 33x56x36 / 13x22x14 |
| Weight, kg/lb | 22/48 |
