『商品說明』
- 適用於細胞培養、動物組織、植物、酵母菌和細菌,試劑中含有 RNase 抑制劑避免萃取過程中造成 RNA 降解 60 分鐘內可完成 Total RNA 萃取,並可去除 DNA 的汙染。
- 適用於 RNA 純化和去鹽,本試劑純化方式可利用離心機純化或搭配 Aurum Vacuum Manifold 使用。
The Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as animal and plant tissues. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a spin-column format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications. The Aurum total RNA mini kit can also be used for RNA cleanup and desalting.
Features include:
- High-quality RNA isolation from a wide variety of samples in less than 60 minutes
- RNase-free reagents and plasticware, including DNase I for removal of genomic DNA, at no additional cost
- Rapid silica binding-based purification technology in a spin-column format
- Easy-to-use spin- or vacuum-compatible protocol
『商品規格』
DNA-free RNA isolation kit, includes RNA binding columns, capless wash and capped microcentrifuge tubes, lyophilized DNase I, RNase-free reagents, for 50 preps
Method
Spin- or vacuum-mediated silica binding
Maximum starting material amounts
20 mg hard animal tissue
40 mg plant or soft to moderately hard animal tissues
2.4 x 109 bacterial cells
Binding capacity
>100 µg purified total RNA
Purity
A260/A280 of 1.9–2.1
RNA size distribution
>200 nucleotides
Preparation time
30–60 min (including DNase I digest)
Maximum elution volume
80 µl (2 elutions of 40 µl each)
Minimum elution volume
30 µl*
54 µg/2.4 x 109 E. coli cells
28 µg/3 x 107 S. cerevisiae cells
28 µg/2 x 106 NIH 3T3cells