『商品說明』
- 適用於細胞培養、酵母菌和細菌,試劑中含有 RNase 抑制劑避免萃取過程中造成 RNA 降解 60 分鐘內可完成 Total RNA 萃取,並可去除 DNA 的汙染。本試劑純化方式可利用離心機純化或搭配 Aurum Vacuum Manifold 使用。
The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a 96-well plate format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.
Features include:
- High-throughput total RNA isolation in less than 60 minutes
- RNase-free reagents and plasticware, including DNase I for removal of genomic DNA at no additional cost
- Rapid silica binding-based purification technology in a 96-well plate format
- Easy-to-use spin- or vacuum-compatible protocol
『商品規格』
DNA-free RNA isolation kit, includes RNA binding plates, collection microplates, lyophilized DNase I, RNase-free reagents, sealing tape, for 2 x 96-well preps
Method
Spin- or vacuum-mediated silica binding
Number of preps
192 (2 x 96-well plate)
Maximum starting material amounts
1 x 106 cultured cells
8 x 108 bacterial cells
2 x 107 yeast cells
Binding capacity
>40 µg purified total RNA
Purity
A260/A280 of 1.9–2.1
RNA size distribution
>200 nucleotides
Preparation time
<60 min (including DNase I digest)
Maximum elution volume
80 µl
Minimum elution volume
30 µl*
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
Yield**
5 µg/8 x 108 E. Coli cells
5 µg/8 x 108 B. cereus cells