abm Rabbit Primary Peripheral Blood Mononuclear Cells T4130

Organism	Oryctolagus cuniculus (New Zealand White Rabbit)
Source Organ	Blood
BioSafety Level	II
Growth Properties	Suspension
Morphology	Spherical
Disease	Normal
Isolation	These cells are extracted from buffy coats using Ficoll-PaqueTM PLUS, sterile Ficoll sodium diatrizoate solution, which separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.
Description	The New Zealand White Rabbit Peripheral Blood Mononuclear Cells (PBMC) consists of lymphocytes and monocytes, which can be used for a variety of immunology applications/assays. The rabbit PBMCs secret cytokines and chemokines to regulate immune response, as well as, provide essential growth supplement for growing rabbit primary cells. These cells are useful in the fields of immunology, infectious disease and autoimmune responses.
Applications	For Research Use Only
Propagation	The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM4130. Rabbit PBMCs do not proliferate in vitro.  Carbon dioxide (CO2): 5%, Temperature: 37.0°C.  To prepare supernatant of cultures of activated T cells, dilute PBMC to 0.5×106 cells per mL in complete growth medium and stimulate with Concanavalin A (10 ug/mL) and PMA (20 ng/mL). Harvest supernatant on days 2, 4 and 6.  To prepare for PBMC plasma cell ELISPOT assay, dilute PBMC to desire concentration and culture in complete growth medium with 0.5 mM 2-Mercaptoethanol (CH044) overnight.
Procedure Overview
Subculturing	The PBMCs do not proliferate in vitro