abm Human Primary Prostate Stromal Mesenchymal Stem Cells (HPS-19I) T4199

Organism	Homo sapiens
Source Organ	Prostate Gland
Donor Gender	Male
BioSafety Level	II
Growth Properties	Adherent
Morphology	Spindle shaped
Disease	Normal
Description	Mesenchymal stem cells are multipotent stromal cells with the ability to differentiate into other cell lineages such as cartilage, bone, and adipose tissues. Tissue-resident mesenchymal stem cells may have a role in repair, fibrosis, cardiovascular diseases, and tumor microenvironment. Studies have suggested that formation of myofibroblasts is associated with diseases such as cancer, fibrosis, and vessel disease. The Human Prostate Stromal Mesenchymal Stem Cells (HPS-19I) are isolated CD44+CD90+ cells from the adult prostate gland which has the ability to differentiate into neurogenic, chondrogenic, and osteogenic cell lineages. In the presence of TGF-β1 the cells differentiate into myofibroblasts by inhibiting RUNX1 expression. The cells potentially are a source of reactive stroma fibroblasts. These cells are recommended in use for stem cell biology, stroma microenvironment, anticancer therapeutic and antifibrotic therapeutic studies.
Materials from	BCM
Applications	For Research Use Only
Markers	CD44, CD90, CD13, CD29, CD73, CD105
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Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM4199.  Carbon dioxide (CO2): 5%, Temperature: 37.0°C.  Note: Density-dependent inhibition is exhibited at more than 70% confluency.
Procedure Overview