abm Human Primary Nucleus Pulposus Cells T4166

Organism	Homo sapiens
Source Organ	Nucleus Pulposus
BioSafety Level	II
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	Normal
Description	The Human Primary Nucleus Pulposus Cells are cryopreserved at passage 2 and can be cultured and propagated for up to 10 doublings before senescence. They are isolated from normal human nucleus pulposus and can be used to study disc degeneration, tissue engineering and cell therapy for spine disc-related disorders.
Applications	For Research Use Only
Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM4166. These cells must be grown in ECM-coated flasks (G299) with the aformentioned medium.  Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Procedure Overview