abm Human Primary Uterine Microvascular Endothelial Cells T4241

Organism	Homo sapiens
Source Organ	Myometrium of Uterine Wall
BioSafety Level	II
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	10,000 - 20,000 cells/cm2
Description	The Human Primary Uterine Microvascular Endothelial Cells are cryopreserved at passage 2 and can be cultured and propagated for up to 10 doublings before senescence. They are isolated from normal human uterine wall, and can be used to study the morphological and biochemical effects in response to sex steroids, or to study relevant diseases.
Applications	For Research Use Only
Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM4241.  Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Procedure Overview
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.