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因中東戰爭造成原物料波動影響,若訂單成立後進貨成本發生重大異動,我們將主動聯繫您協商處理。造成不便,敬請見諒。
Organism |
Newborn Fisher rats |
Source Organ |
Cerebral cortex |
BioSafety Level |
II |
Growth Properties |
Adherent |
Morphology |
Fibroblast-like |
Description |
The Enhanced Primary Rat Cerebral Cortex Cells (HO Clones) exhibit a unique morphology in which the cells when forming a colony, exhibit a hole in the center. They have been infected with a retroviral vector containing the avian Myc gene (pneoMLV), but are not immortal. These cells express GFAP and GalC (a marker for oligodendroglial lineage) markers. |
Applications |
For Research Use Only |
Image |
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Propagation |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 5% horse serum, 0.3 mg/ml G418 (G271), and Penicillin/Streptomycin (G255).Carbon dioxide (CO2): 5%, Temperature: 37.0°C. |
Procedure Overview |
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Quality Control |
Immunofluoresence analysis for markers |
