abm Mouse Primary Cochlear Perivascular Resident Macrophate-type Melanocytes (PVM/Ms) T4575

Organism	C57BL/6J mice
Source Organ	Cochleae
Donor Age	10- to 15-day-old
BioSafety Level	II
Growth Properties	Adherent
Morphology	Melanocyte-like
Disease	Normal
Isolation	Mouse Primary Perivascular Resident Macrophate-type Melanocytes (PVM/Ms) were isolated and purified by flow cytometry based on F4/80 sorting. Sorted cells were validated with anti-F4/80 and anti-GSTα4 in immunohistochemical staining.
Description	As part of the fluid-blood barrier in the stria vascularis, the perivascular resident macrophate-type melanocytes (PVM/Ms), along with cochlear microvascular endothelial cells and cochlear pericytes, are important for maintaining the inner ear homeostasis. The PVM/Ms have been found to be essential in regulating the permeability of the intrastrial fluid-blood barrier. With all three primary cells available, co-culture of the cell types is useful in studying the underlying causes of vascular dysfunction-related hearing loss.
Applications	For Research Use Only
Markers	F4/80, CD68, and CD11b
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Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is 254CF Medium (Invitrogen, Cat No. M254CF500). To make the complete growth medium, add the following components to The base medium: 10% fetal bovine serum (TM999), 1% human melanocyte growth factor (Life Technologies, Cat. No. S-002-5) and Penicillin/Streptomycin (G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%; Temperature: 37.0°C.
Procedure Overview