abm Mouse Primary Cochlear Pericytes T4576

Organism	C57BL/6J mice
Source Organ	Cochleae
Donor Age	10- to 15-day-old
BioSafety Level	II
Growth Properties	Adherent
Morphology	Large|Stellate shaped
Disease	Normal
Isolation	Mouse Primary Pericytes were isolated and purified by flow cytometry based on platelet-derived growth factor beta receptor sorting. Sorted cells were validated with Anti-desmin and Anti-NG2 antibodies in immunohistochemical staining.
Description	As part of the fluid-blood barrier in the stria vascularis, the cochlear pericytes, along with cochlear microvascular endothelial cells and perivascular resident macrophate-type melanocytes (PVM/Ms), are important for maintaining the inner ear homeostasis. Studies have shown that cochlear pericytes regulate the tight junction in the endothelial cell monolayer. With all three primary cells available, co-culture of the cell types is useful in studying the underlying causes of vascular dysfunction-related hearing loss.
Applications	For Research Use Only
Markers	Desmin, PDGFRβ, NG2
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Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available in abm, Cat. No. TM003. To make the complete growth medium, add the following components to The base medium: 10% fetal bovine serum (TM999), 100 nM pigment epithelium-derived factor (PEDF, abm Cat. No. Z100765) and Penicillin/Streptomycin (G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%; Temperature: 37.0°C.
Procedure Overview