abm Mouse Primary Cochlear Endothelial Cells T4577

Organism	C57BL/6J mice
Source Organ	Cochleae
Donor Age	10- to 15-day-old
BioSafety Level	II
Growth Properties	Adherent
Morphology	Flat|Elongated|Cuboidal
Isolation	Mouse Primary Cochlear Endothelial Cells were isolated and purified by flow cytometry based on isolectin B4 (BSI-B4) sorting. Sorted cells were validated with anti-von Willebrand factor (vWF) antibody in immunohistochemical staining.
Description	As part of the fluid-blood barrier in the stria vascularis, the cochlear microvascular endothelial cells, along with perivascular resident macrophate-type melanocytes (PVM/Ms) and cochlear pericytes, are important for maintaining the inner ear homeostasis. The cochlear microvascular endothelial cells form tight junctions and primarily sustains the barrier integrity. With all three primary cells available, co-culture of the cell types is useful in studying the underlying causes of vascular dysfunction-related hearing loss.
Applications	For Research Use Only
Markers	vWF
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Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is CS-C Medium (Sigma, Cat No.C1556). To make the complete growth medium, add the following components to The base medium: 10% fetal bovine serum (TM999), 1% endothelial cell growth factor (Sigma, Cat. No. E9640) and Penicillin/Streptomycin (G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%; Temperature: 37.0°C.
Procedure Overview