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HIMEDIA Luria Bertani Agar, Miller (Miller Luria Bertani Agar) M1151
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選購型號
商品介紹
HIMEDIA® Luria(Lysogeny)Bertani Agar Miller (LB)(CALK0019-M1151-500EA-2.pdf)
HIMEDIA® Luria(Lysogeny)Bertani Agar Miller (LB)(CALK0019-M1151-500EA-1.pdf)
用於遺傳和分子研究的大腸桿菌重組菌株的培養和維護，也可用於普通微生物的常規分離培養
Intended Use
Recommended for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular biology studies; may be used for routine cultivation and estimation of not particularly fastidious microorganisms.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 10.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 10.000 |
| Agar | 15.000 |
Final pH (at 25°C): 7.5±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired. Mix well and and pour into sterile Petri plates.
Principle And Interpretation
Luria Bertani Agar is prepared as described by Lennox (4) for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller (1) is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media.
Tryptone provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.
Type of specimen
Recombinant strains of E.coli
Specimen Collection and Handling
For Recombinant strain samples follow appropriate techniques for handling specimens as per established guidelines (1,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some strains may show poor growth due to nutritional variations.
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance Cream to yellow homogeneous free flowing powder
Gelling Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow to amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.0% w/v aqueous solution at 25°C. pH : 7.5±0.2
pH
7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% |
| Escherichia coli DH5 alpha MTCC 1652 | 50-100 | luxuriant | >=70% |
Key: (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
Reference
- Atlas R.M., 1993, Handbook of Microbiological Media, Ed. by Parks L., CRC Press, Inc.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
- Lennox E.S., 1955, Transduction of Linked Genetic Characters of the host by bacteriophage P1., Virology, 1:190.
