Norgen Cell Culture Media Exosome Purification Midi Kit 60500

Cell Culture Media Exosome Purification Kits The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen's proprietary resin. These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression. NanoSight® Analysis Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration Exosomal RNA Analysis Exosomal RNA can be isolated using Norgen's Exosomal RNA Isolation Kit from exosomes enriched using Norgen's Cell Culture Media Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for biomarker discovery. Exosomal RNA isolation is based on Norgen's proprietary resin without the need for phenol extractions or carrier RNA. • Purification and enrichment of intact cell culture media exosomes for functional studies • Versatile sample input ranging from 5 mL to 35 mL o Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL) o Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL) o Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL) • No time-consuming ultracentrifugation, filtration nor special syringes required • No precipitation reagents or overnight incubation required • No protease treatment required • Compatible with cell culture media from any species • Pure exosomes are purified and are free-from any other RNA-binding proteins • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration