Norgen Sputum DNA Isolation Kit - 25 Preps 46200

Sputum DNA Isolation Kit This kit constitutes an all-in-one system for the rapid isolation of DNA from sputum samples. It allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using a convenient spin column format. The kit includes all the reagents needed to process sputum DNA including a sputum liquifier to reduce viscosity and facilitate DNA isolation. The protocol can be completed in 30 minutes. Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR. Background A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis, Mycobacterium tuberculosis, Streptococcus pneumoniae and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation. • Fast and easy processing using a spin column format • DNA can be isolated and detected from as little as 100 µL of sputum • Effective removal of PCR inhibitors • Fully compatible with Norgen’s Sputum DNA Collection, Preservation and Isolation Kit (Cat#RU46100).