Norgen BAC DNA Kit, various volumes new format 18050

BAC DNA Miniprep Kit This kit is designed for the rapid preparation of BACs (bacterial artificial chromosomes) and other large DNA constructs from small batch cultures of Escherichia coli. Purification of constructs up to 130 kb in size have been verified. Rapid alcohol precipitation or spin-column purification allows for the processing of multiple samples in 1 hour. The DNA is preferentially purified from other cellular components such as genomic DNA and RNA. Typical DNA recoveries range between 0.6 µg and 1 µg from 3.0 mL of bacterial cultures. The purified DNA is fully digestible with all restriction enzymes tested, and is fully compatible with bacterial transformation and sequencing to achieve 95-100% accuracy. • Fast and easy processing using alcohol precipitation or a rapid spin-column format • High yield of plasmid DNA – up to 1 µg from 3 mL of culture • Versatile protocol that allows different volume of input to be processed