Norgen AAV Purification Kit 66100

AAV Purification Kit Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments. Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. • AAV Purification from any input – cell fraction or media fraction • Purification from 500 mL of cell culture supernatant or from ~15 mL of cell pellet per kit • Rapid purification within 2 to 4.5 hours • High AAV recovery, up to 90% • No specialized equipment needed • Purification from a variety of AAV serotypes (including AAV6 and AAV9) • Purify AAV cell culture supernatant from 1 mL to 33.5 mL input per prep • Purify AAV cell pellet from 1 mL of input per prep • Yields highly active AAV for in vivo and in vitro experiments • Up to 33X sample concentration