The E.Z.N.A.® Total RNA Midi Kit is designed for RNA isolation from large amounts of sample. The E.Z.N.A.® Total RNA Midi Kit can purify total RNA from up to 1×108 cultured cells or 200 mg animal tissues. By using the HiBind® midi column technology, the E.Z.N.A.® Total RNA Midi Kit greatly reduces processing time when compared to traditional methods such as CsCl ultracentrifugation or phenol/chloroform protocols. Purified RNA can be used in downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.
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