pVectOZ Transfection Plasmids are engineered in an optimized backbone and are ideal for all transfections. OZ Biosciences proposes five expressions vectors encoding for the most popular reporter genes (CAT, GFP, LacZ, Luciferase, SEAP)
pVectOZ- encoding genes:
1. Chloramphenicol Acetyltransferase (CAT) – pVectOZ-CAT
2. Green Fluorescent Protein (GFP) – pVectOZ-GFP
3. beta-Galactosidase (Lac Z) – pVectOZ-LACZ
4. Luciferase (Luc) – pVectOZ-LUC
5. Secreted Alcaline Phosphatase (SEAP) – pVectOZ-SEAP
The transgene expression level depends mainly on the promoter, enhancer, terminator and plasmid backbone. The pVectOZ expression cassette was designed to express transgene product in many mammalian cells and tissues. This vector has been modified to eliminate sequences affecting transgene expression levels while optimizing those critical for the high levels of expression. The final expression cassette accommodates the high levels of transgene expression in mammalian cells as well as high yield of plasmid production in Escherichia coli. The resulting plasmid is the ideal vector for expression in vitro and in vivo.
pVectOZ main features:
1. Highest levels of transgene expression in mammalian cells and tissues
2. Suitable for all transfection applications (in vivo & in vitro)
3. High copy number production in Escherichia coli
4. LPS-endotoxin free, supercoiled and highly purified: transfection grade approved
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