OZ Biosciences ONPG assay kit is ideal to quantify high expression level of beta-Gal. The levels of active beta-Gal expression can be quickly measured by its catalytic hydrolysis of ONPG (o-nitrophenyl-beta-D-galactopyranoside) substrate to a bright yellow product (Absorbance at 405-420nm).LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, beta-Gal), is very stable, resistant to proteolytic degradation and easily assayed.
All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells.
1. Convenient for all transfection assays
2. Suitable for cultured cells and tissues
3. Simple, rapid and easy-to-use
4. Economical
5. Optimized with stable buffers
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