OZ Bioscience ROS DETECTION ASSAY KIT (300 ASSAYS) 活性含氧物檢測套組 ROS0300

The ROS Detection Assay kit (Green Fluorescence) is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA). Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell. Main Features 1. Measure ROS production in live cells 2. Ideal for fluorescence microscopy, microplate titration and cytometry 3. Compatible with adherent cells as well as suspension cells