CaLiVax-DOTAP is a cationic lipid-based composition for liposome-mediated DNA or protein vaccines. Cationic Liposome-DOTAP Adjuvant: average size of 100 nm. DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N trimethyl-ammonium methyl-sulfate):Cholesterol (1:1 molar ratio) cationic lipid composing this genetic adjuvant allows the formation of complexes with plasmid DNA or antigen protein to form an efficient lipoplexes-based nanoparticle delivery system (LPD). LDP are non-viral gene delivery systems, self-assembled from cationic liposomes and negatively charged immunogen that function as vaccine carrier.