Description
The TaqMan® Fast Reagents Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5’ nuclease assay, in fast thermal cycling mode. The Eukaryotic 18S rRNA TaqMan® Assay consists of a 20X mix of two unlabeled PCR primers and a FAM™ dye labeled 18S TaqMan® MGB probe.
Used With:
7900HT Fast Real-Time PCR System with Fast 96-Well Block Module, 7900HT Fast Real-Time Service Upgrade, 7900HT System Fast 96-Well Block Upgrade Kit.
For Research Use Only. Not for use in diagnostics procedures.
Contents & storage
This kit contains TaqMan® Fast Real-Time PCR Universal Master Mix (2X), no AmpErase® UNG (1 tube) , Eukaryotic 18S rRNA TaqMan® Assay (20X mix of two unlabeled PCR primers and a FAM™ dye-labeled 18S TaqMan® MGB probe) (Assay ID Hs99999901_s1; Part Number 4331182), and human Raji cDNA.
Store the PCR mix at 2 - 8°C. Store the rest of the kit contents at -15 to -25°C.
Specifications
| Detection Method: |
Primer-Probe Detection |
| For Use With (Equipment): |
7500 Fast System, 7900HT Fast System |
| Reaction Speed: |
Fast |
| PCR Method: |
qPCR |
| Sample Type (General): |
DNA |
| Hot Start: |
No |
| Passive Reference Dye: |
ROX (Pre-mixed) |
| Polymerase: |
AmpliTaq® DNA Polymerase |
| Primer-Probe Compatibility: |
TaqMan® |
| Product Line: |
TaqMan™ |
| Product Size: |
125 reactions |
| Sample Type (Specific): |
DNA (Genomic), cDNA |
| Shipping Condition: |
Dry Ice |
| Target Gene: |
18S rRNA |
| Target Specificity: |
Target-Specific |