Description
Applied Biosystems® TaqMan® Brucella spp. Detection Kit uses the polymerase chain reaction (PCR) to amplify a unique microorganism specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.The TaqMan® Brucella spp. Detection Kit provides a simple reliable and rapid procedure that utilizes the polymerase chain reaction (PCR) to amplify a target sequence unique to Brucella spp.
• Reduce sample handling with a 3-step process that uses a single closed-tube format during the PCR step.
• Highly sensitive TaqMan® Real-Time PCR system enables a dramatic reduction in time required for pre-enrichment compared to conventional techniques.
• Streamline your workflow with one standard protocol for multiple organisms.
3 Easy Steps
This kit has 3 easy steps: enrich samples prepare samples and run samples. Grow the specific pathogen from a selected sample using a standard enrichment procedure in the enrichment step. In the sample preparation step isolate the DNA for PCR and prepare mix and pipet the reactions. In the final step analyze your sample by running a plate on one of our real-time PCR systems.
A Complete Solution
We offer a full line of TaqMan® Pathogen Detection kits based on universal assay conditions enabling you to run any combination of these assays together in the same sample run. In combination with our real-time PCR systems the TaqMan® Brucella spp. Detection Kit offers a fully integrated single-vendor solution to identify Brucella spp.
Contents & storage
300µL 10X Brucella spp. Assay Mix (store at -20°C) 1 000µL Negative Control (store at 2°C-8°C) 2X Fast PCR Master Mix 1 500µL (store at 2°C-8°C).
Specifications
| Target Organism Class: |
Brucella spp. |
| Strain or Genotype: |
Brucella spp. |
| Validated Application: |
Real-Time PCR |
| Detection Method: |
Primer-Probe Detection |
| Disease: |
Brucellosis |
| Label or Dye: |
FAM |
| Product Line: |
TaqMan™ |
| Product Size: |
100 reactions |
| Shipping Condition: |
Dry Ice |
| Target Specificity: |
Target-Specific |