Description
This Taqman® assay provides a simple reliable and rapid procedure for detecting the presence of a specific bacterial or viral target. PCR is used to amplify a DNA target unique to the microorganism then TaqMan® probe is used to detect and quantify the target in a easy-to-use single tube format. This assay enables microbial detection capabilities with high-confidence results to will help accelerate your food and environmental testing needs. The assay design is based on "Detection and Isolation of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) from Meat Products" method #5B.03 from the Food Safety and Inspection Service (FSIS) Molecular Laboratory Guidebook (MLG). The assay has been optimized for use with the included Environmental Master Mix; a negative control is not included with this assay.
Features of TaqMan® assays:
• Include an internal positive control to monitor your PCR
• Suitable for single or multiplexed assays
• Easy-to-use single tube format
• Run using universal conditions- analyze multiple assays in the same run
• Robust assay design provides highly accurate reproducible and reliable results
• Include passive internal reference based on proprietary ROX™ dye for precise data analysis
This product is for environmental testing quality control/quality assurance testing food and agricultural testing and research purposes only.
Custom assays have been bioinformatically developed and tested for performance. Validation services for specific samples types are provided by our Custom Solutions team. Please contact your local Thermo Fisher Scientific representative for more information.
Contents & storage
• 10x Assay Mix (Store at -5°C to -30°C.)
• 2x Environmental Master Mix (Store at -5°C to -30°C upon receipt. Store at 2–8°C after use.)
These reagents are sufficient for 100 reactions.
Specifications
| Comments: |
Includes Master Mix |
| Form: |
Liquid |
| Recommended Applications: |
Polymerase Chain Reaction |
| Target Organism Class: |
E. coli |
| Detection Method: |
Primer-Probe Detection |
| Reaction Speed: |
Standard |
| Product Size: |
100 reactions |
| Species: |
All Species |