Thermo Fisher MLG TAQMAN STEC O26 & O111 4485064

Description

These TaqMan® assay beads provide a simple reliable and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan® probe to detect and quantify the target all in an an easy-to-use lyophilized bead format. TaqMan® assay beads deliver the fastest RT-PCR run times on the market (<40 minutes in Fast mode). Simply add 30 µL of purified DNA to the beads and run on Fast or standard instrumentation. These TaqMan® assay beads provide microbial detection capabilities and high-confidence results to help accelerate your food and environmental testing needs. The assay design is based on "Detection and Isolation of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) from Meat Products" method #5B.03 from the Food Safety and Inspection Service (FSIS) Molecular Laboratory Guidebook (MLG).

Features of TaqMan® assay beads:

• Convenience—simply add your nucleic acid sample to the bead-containing assay tube and run; no pre-mixing or master mix preparation required
• Consistency—fewer operator steps means reduced error and increased reproducibility across time users and laboratories
• Improved data accuracy—an internal positive control is included to monitor your PCR
• Fast chemistry—40-min run time with Fast cycling compatible with standard or Fast modes

TaqMan® assay beads are suitable for single or multiplex analysis. Because they are run using universal conditions multiple bead-based assays can be analyzed in the same run. The robust assay design provides highly accurate reproducible and reliable results with the enhanced stability of a lyophilized format. A passive internal reference based on proprietary ROX™ dye is included for precise data analysis.

This product is for environmental testing quality control/quality assurance testing food and agricultural testing and research purposes only.

Contents & storage

Specifications

Comments:	Includes Master Mix
Form:	Lyophilized
Target Organism Class:	E. coli
Validated Application:	Polymerase Chain Reaction
Detection Method:	Primer-Probe Detection
Reaction Speed:	Fast  Standard
Product Size:	96 reactions
Species:	All Species