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Thermo Fisher SALMONELLA STARTER KIT WITH PREPSEQ NUCLEIC ACID EXTRACTION KIT 4412639
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商品介紹
Description
The MicroSEQ Salmonella Species Detection Starter Kit with PrepSEQ Nucleic Acid Extraction Kit is recommended for those needing to prepare and analyze hundreds of food or environmental samples per day. The Starter Kit detects Salmonella species simply reliably and rapidly using the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan probe to detect the amplified sequence. The MicroSEQ Salmonella Species Detection Kit is optimized for use with the PrepSEQ Nucleic Acid Extraction Kit. For ease of ordering we have combined a 96 reaction MicroSEQ Salmonella Species Detection Kit with a 100 reaction PrepSEQ Nucleic Acid Extraction Kit and offer it here as a single product.Features of the MicroSEQ Salmonella Species Detection Starter Kit:
• Enjoy ease-of-use from a streamlined lyophilized format and a simple software-guided 3-step process
• Time-to-results of less than 3 hours after sample enrichment (less than 19 hours total)
• Eliminate false negatives with internal positive control
• Detect 1–3 colony-forming units (CFU) per 25 grams of food
• Universal PCR cycling conditions for all MicroSEQ pathogen detection kits
• Validated by the AOAC Research Institute
Optimized Sample Preparation
The MicroSEQ Salmonella Species Detection Kit is optimized for use with PrepSEQ Nucleic Acid Extraction Kit to produce high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples for detection of 1–3 cfu per 25 grams of sample. In combination with either the automated MagMAX Express-96 Deep Well Magnetic Particle Processor or the KingFisher Flex Purification System with KingFisher 96 Deep-well Head this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.
Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results the reagents used in the MicroSEQ Salmonella Species Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme the target-specific primer and probe set Internal Positive Control (IPC) and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.
Universal Fast PCR Cycling Conditions
All MicroSEQ pathogen detection kits utilize universal PCR cycling conditions which reduce error and allow maximum user flexibility. Fast PCR chemistries are completed in 40 minutes—saving time and clearing presumptive negatives quickly.
Clear Results using a Software-Guided Procedure
After sample enrichment and preparation RapidFinder Express Software (available separately) guides you through the entire workflow with on-screen instructions. Amplification detection data collection and analysis are fully automated. When the assay is complete the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags notifications and prompts that enable you to quickly interpret the data and take appropriate action.
AOAC Certified
The full workflow including the MicroSEQ Salmonella Species Detection Kit PrepSEQ Nucleic Acid Extraction Kit 7500 Fast instrument and RapidFinder Express software has earned the Performance Tested Methods certification from the AOAC Research Institute.
A Complete Solution
The MicroSEQ Salmonella Species Detection Starter Kit with PrepSEQ Nucleic Acid Extraction Kit is part of a complete food testing solution. The kit includes the reagents required to prepare up to 100 sample preparations and run 96 Salmonella species detection assays. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Salmonella species detection as fast easy and reliable as possible.
For testing of Food and Environmental samples only.
Contents & storage
Detection Kit
• 12 8-tube strips containing assay beads (store at 2 to 8°C)
• Optical 8-tube strips and 8-cap strips (store at room temperature)
• Negative Control (store at 2 to 8°C)
• Protocol and Quick Reference Card
The detection kit is sufficient for 96 assays.
Extraction Kit
• 2 bottles of Lysis Buffer (store at room temperature)
• 2 tubes of Magnetic Particles (store at 2 to 8°C)
• 1 empty bottle for binding solution (store at room temperature)
• 1 bottle of Wash Buffer Concentrate (store at room temperature)
• 1 bottle of Elution Buffer (store at room temperature)
• 1 bottle of Proteinase K Buffer (store at room temperature)
• 1 tube of Proteinase K (store at -20°C)
The extraction kit is sufficient for 100 extractions.
• 12 8-tube strips containing assay beads (store at 2 to 8°C)
• Optical 8-tube strips and 8-cap strips (store at room temperature)
• Negative Control (store at 2 to 8°C)
• Protocol and Quick Reference Card
The detection kit is sufficient for 96 assays.
Extraction Kit
• 2 bottles of Lysis Buffer (store at room temperature)
• 2 tubes of Magnetic Particles (store at 2 to 8°C)
• 1 empty bottle for binding solution (store at room temperature)
• 1 bottle of Wash Buffer Concentrate (store at room temperature)
• 1 bottle of Elution Buffer (store at room temperature)
• 1 bottle of Proteinase K Buffer (store at room temperature)
• 1 tube of Proteinase K (store at -20°C)
The extraction kit is sufficient for 100 extractions.
Specifications
| Target Organism Class: | Salmonella spp. |
|---|---|
| Strain or Genotype: | Salmonella spp. |
| Validated Application: | Real-Time PCR |
| Detection Method: | Primer-Probe Detection |
| PCR Method: | qPCR |
| For Use With (Equipment): | 7500 Fast System KingFisher™ Flex MagMAX™ Express-96 Deep Well Magnetic Particle Processor StepOne™ Fast Mode StepOnePlus™ Fast Mode |
| Sample Type (Specific): | Any Swab Type Food Water Samples |
| Product Size: | 96 assays |
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