Product Introduction
3D Tissue Homogenizer (-40℃) adopts a special three-dimensional integrated operation mode. The sample moves in a 3D "∞" shape in space, and completes the crushing of the sample in a very short time. It is suitable for research institutes, universities, agricultural institutes, biological Special equipment for one-time rapid processing of multiple samples in the fields of medicine and food testing.
The 3D Homogenizer Frozen Sample Grinding largely avoids the degradation of nucleic acids and the denaturation of proteins. The grinding process generates less heat, has almost no destructive effect on nucleic acids and proteins, and has high integrity. It is suitable for library building, cloning and sequencing of whole genes.
A wide variety of samples can be processed:
a. Suitable for grinding and crushing of various plant tissues including roots, stems, leaves, flowers, fruits, seeds and other samples;
b. Suitable for grinding and crushing of various animal tissues including brain, heart, lung, stomach, liver, thymus, kidney, intestine, lymph node, muscle, bone and other samples;
c. Suitable for grinding and crushing of cells and microorganisms;
d. Grinding and crushing suitable for the analysis and detection of food and drug components;
A closed disposable centrifuge tube is used, which can effectively avoid cross-contamination between samples;
The experiment has strong repeatability, and the same grinding frequency and time can be set to obtain the same grinding effect;
Compressor refrigeration function: -40°C to room temperature; Temperature control accuracy: ±1°C;
Product Features
1. Special ∞ shaped operation mode for fuller grinding;
2. Particularly suitable for tough samples, grinding can be completed in 60s, while conventional tissues can be ground in as little as 15s;
3.Minimum refrigeration temperature -40℃, the whole process of low-temperature grinding, reduce protein and RNA degradation;
4.Up to24 samples can be processedat the same time;
5.A variety of adapters to choose from.
Technical Parameters
1. It can process up to 24 samples at the same time within 15 seconds, and complete sample grinding quickly, efficiently and with high throughput;
2. Using a special three-dimensional integrated operation mode, the sample moves in a three-dimensional "∞" shape in space, with efficient grinding and better stability;
3. A wide range of samples can be processed, plant tissues (such as rice, corn, wheat, beans, peanuts, etc.), animal tissues (such as muscle, small intestine, heart, lung, fur, bone, etc.), microorganisms and various Chinese medicinal materials, medicines, etc.;
4. The grinding process adopts a closed disposable centrifuge tube, which effectively avoids cross-contamination between samples;
5. Preset 10 groups of common tissue grinding parameters to optimize grinding conditions for different specimens;
6. Homogenizing speed: 3m/s-8m/s, the actual speed can reach 4500rpm, working time: 0 seconds-99 minutes, the user can set it;
7. Maximum feed size: no requirement, adjusted according to the adapter;
8. Emergency stop button: It can be photographed at any time during the grinding process, and the instrument will stop running immediately, which is fast and safe;
9. Electromagnetic safety lock: screen click control, automatically unlock when power off; the cover cannot be opened during the working process until the end of the grinding process, and the whole process is protected;
10. The knob-type gland fixes the grinding tube, and the safety lock prevents the thread from slipping and loosening, which makes the sample placement safer and the fixation more reliable.
11. Provide no less than 60 SCI cited papers as experimental guidance
12. Grinding method: wet grinding, dry grinding, low temperature grinding;
13. Compressor refrigeration: cool down to below zero within 10 minutes of starting the machine, temperature range: -40°C - room temperature;
14. Noise level: <65db;
15. Dimensions: 500*365*370mm.
Parameter Setting and Grinding Bead Reference
Product Name | Grinding Beads | Speed | Grinding Time | Pause Time | Cycle times |
Plant Leaves | 2mm*8 | 6m/s | 30s | 15s | 2 |
Heart, Liver, Spleen, Lungs, Kidneys, Brainof Miceand Rats | 2mm*8 | 6m/s | 30s | 10s | 1 |
Stomach, Intestines, Muscles, Fat, Pancreasof Mice and Rats | 2mm*10 | 6m/s | 30s | 15s | 2 |
Skin, Ears, Tail, Blood Vessels of Miceand Rats | 2mm*12 | 7m/s | 45s | 20s | 3 |
Cartilage, Hair, Hard Bones of Miceand Rats | 3mm*1 | 7m/s | 45s | 20s | 3 |
2mm*12 | |||||
(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.) |
For RNA extraction, the grinding time is extended compared with that of protein extraction, and RNA extraction solution is added to the grinding, the specific grinding time depends on the amount and type of tissue to be ground. Samples difficult to break can be repeated grinding 2-3 times, the specific situation can be adjusted appropriately according to the experiment.
Strong and ToughTissue Grinding
For samples that are difficult to grind (e.g. cartilage, hair, etc.), we recommend using a 2mL grinding tube. Cut the sample into 1-2mm pieces, add the appropriate number of grinding beads, cover the tube tightly and freeze in liquid nitrogen for 2-3min without extracting solution, dry grind for 60 seconds at 7m/s, then add extracting solution to the tube and repeat the grinding once. If the grinding result is not satisfactory, the tube can be frozen again and the above experimental steps can be repeated.
Parameters Comparison
Cat. No. | KZ-5F-3D |
Operating mode | Move in a "∞" shaped three-dimensional motion in space. |
Temperature | -40℃ |
Temperature control precision | ±1℃ |
Standard spec. | 24*2 mL |
Maximum sample volume | 24 pcs |
Adapter specification | 24*2mL. 12*5mL(Optional ) |
Freezing table | N/A |
Adapter fixing method | Knob cover with safety locking device |
Working time | 0-99min99s |
Dimensions | 370*365*500mm |
Screen size | 5 inches |
Emergency stop button | YES |
Electromagnetic safety lock | Screen click control with automatic power-off unlocking. the cover cannot be opened during operation until the grinding program ends, fully protected. |
Maximum feeding size | No requirements, adjustable according to the adapter. |
Weight | 52kg |
Power parameters | 200-240V AC,50-60 Hz, |
400 W | |
Cooling method | Compressor cooling |
Transport screws | N/A |
Advantages | Higher grinding efficiency, reducing tissue degradation.Freezing function, lowest temperature can reach -40℃. |