Servicebio 冷凍組織研磨機 KZ-III-FP

Product Introduction
KZ-Ⅲ-FP  Lowtemperaturetissuehomogenizer (-40℃) can reach a low temperature environment of -40 °C. It uses a vertical oscillation system to reciprocate at high speed to make the frozen sample in the grinding tube collide with the grinding beads. The tissue is completely broken, and it is a special equipment for one-time rapid processing of multiple samples in research institutes, universities, agricultural institutes, biomedicine, food testing and other fields.

Traditional mortar grinding can only grind one sample at a time, and the processing efficiency is low; and tools such as the mortar must be cleaned and sterilized each time, which greatly increases the workload of sample processing.

Low Temperature Homogenizer Sample Grinding -40℃ can greatly improve sample processing:

1. Dry grinding, wet grinding and homogenization of samples can be performed;

2. It can process up to 24 samples at the same time (upgradable to process 60 samples at the same time), and complete the sample preparation in a fast and high-throughput manner;

3. Can be replaced with different capacity adapters 0.5ml, 2ml, 5ml, 10ml, 50ml;

4. Wide range of samples that can be processed:

a. Suitable for grinding and crushing of various plant tissues including roots, stems, leaves, flowers, fruits, seeds and other samples;

b. Suitable for grinding and crushing of various animal tissues including brain, heart, lung, stomach, liver, thymus, kidney, intestine, lymph node, muscle, bone and other samples;

c. Grinding and crushing suitable for the analysis and detection of food and drug components;

5. A closed disposable centrifuge tube is used, which can effectively avoid cross-contamination between samples;

6. The experiment has strong repeatability, and the same grinding frequency and time can be set to obtain the same grinding effect;

7. Refrigeration function: -40℃ to room temperature; Temperature control accuracy: ±1℃.

Product Features

1. Slider drive, no wear shaft, noise stability and durability;

2. Minimum refrigeration temperature -40℃, the whole process of low-temperature grinding, reduce protein and RNA degradation;

3.Up to 60 samples can be processed simultaneously in 1min (24 samples come standard);

4.A variety of adapters to choose from.

Technical Parameters

1. It can process up to 24 samples at the same time in 1 minute (upgrade to process 60 samples at the same time), and complete the sample grinding quickly, efficiently and with high throughput;

2. Large touch screen design, easy to operate, and 10 groups of common tissue grinding parameters (plant stem and leaf, tissue heart, liver, spleen, lung and kidney, skin, bone, etc.) are preset to optimize the grinding conditions of different specimens;

3. Noise level: <65db;

4. Maximum feed size: no requirement, adjusted according to the adapter;

5. Emergency stop button: It can be photographed at any time during the grinding process, and the instrument will stop running, which is fast and safe;

6. Rapid cooling: cool down to below zero in a short time after starting to prevent degradation, temperature range: -40°C - room temperature;

7. Electromagnetic safety lock: the electromagnetic lock cannot open the cover during the working process, until the end of the grinding process, and the whole process is protected;

8. Grinding method: wet grinding, dry grinding, low temperature grinding;

9. Provide no less than 60 SCI cited papers as experimental guidance

10. Using a special three-dimensional vibration mode of up and down and left and right shaking, the grinding is more sufficient and the stability is better;

10. An optional 60-hole adapter can be installed, which can be upgraded to grind 60 samples each time, further improving the grinding efficiency.

11. Different adapters can be selected to match different requirements for sample grinding: 24*0.5ml; 48*2ml; 32*2ml; 60*2ml; 12*5ml; 10*10ml; 4*30ml; 2*50ml (also according to Customized according to actual needs)

12. Dimensions: 500*365*370mm

Parameter Setting and Grinding Bead Reference

1) Protein Extraction

2) RNA Extraction 

For RNA extraction, the grinding time is extended compared with that of protein extraction, and RNA extraction solution is added to the grinding, the specific grinding time depends on the amount and type of tissue to be ground. Samples difficult to break can be repeated grinding 2-3 times, the specific situation can be adjusted appropriately according to the experiment.

Strong and Tough Tissue Grinding

For samples that are difficult to grind (e.g. cartilage, hair, etc.), we recommend using a 2mL grinding tube. Cut the sample into 1-2mm pieces, add the appropriate number of grinding beads, cover the tube tightly and freeze in liquid nitrogen for 2-3min without extracting solution, dry grind for 60 seconds at 70Hz, then add extracting solution to the tube and repeat the grinding once. If the grinding result is not satisfactory, the tube can be frozen again and the above experimental steps can be repeated.

Bone Tissue Grinding

For bone tissue, use the special steel grinding jar and adapter. The bone tissue is first frozen in liquid nitrogen for 5-10min, then put into the grinding jar, add the steel beads, and grind for 180 seconds at 70Hz (set cycle grinding). After grinding, take out the finished tissue, put it into a 2mL grinding tube, add the extraction solution, two 3mm grinding beads and homogenize for 60 seconds.

 Parameters Comparison