The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can run 1–12 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the isoelectric focusing tray is independently powered, enabling precise control over each IPG strip. This feature makes it possible to run different sample types, different pH gradients, and multiple protocols simultaneously.
Learn more about isoelectric focusing with the PROTEAN i12 IEF system.
Features and Benefits of the PROTEAN i12 IEF System
Optimize experiments in fewer runs — the PROTEAN i12 IEF system has the unique ability to run channels under multiple conditions in a single run. Different samples, protocols, and pH gradients can be run simultaneously.
Obtain higher-quality data with less experimental risk — each channel is independent, so one irregular sample can no longer compromise the entire run.
Easy, intuitive programming — easily run, create, and edit isoelectric focusing protocols from the touch screen user interface. Preprogrammed protocols stored in the internal memory serve as a convenient starting point for developing optimized sample-specific IEF conditions.
Run gel side up, gel side down, or with loading cups — focusing trays and electrode assemblies accommodate all possible gel configurations.
Applications and Uses of the PROTEAN i12 IEF System
Isoelectric focusing is the first dimension of 2-D electrophoresis, a powerful technique used for the visualization and identification of proteins. The PROTEAN i12 IEF system can be used for a number of applications including the following: